In vivo assembly of DNA-fragments in the moss, Physcomitrella patens
نویسندگان
چکیده
Copenhagen Plant Science Centre, VILLUM Research Center for Plant Plasticity, Center for Synthetic Biology “bioSYNergy”, Department of Plant and Environmental Sciences, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia Department of Systems Biology, Technical University of Denmark, Søltofts Plads, 2800 Kgs. Lyngby, Denmark
منابع مشابه
Corrigendum: In vivo assembly of DNA-fragments in the moss, Physcomitrella patens
Direct assembly of multiple linear DNA fragments via homologous recombination, a phenomenon known as in vivo assembly or transformation associated recombination, is used in biotechnology to assemble DNA constructs ranging in size from a few kilobases to full synthetic microbial genomes. It has also enabled the complete replacement of eukaryotic chromosomes with heterologous DNA. The moss Physco...
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This method is adapted from a protocol described by Grimsley et al. (1977). For more information about P. patens as a model organism, see The Moss Physcomitrella patens: A Novel Model System for Plant Development and Genomic Studies (Cove et al. 2009a). For details about the growth of P. patens on cellophane overlay plates, see Culturing the Moss Physcomitrella patens (Cove et al. 2009b). For p...
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RELATED INFORMATION For more information about P. patens as a model organism, see The Moss Physcomitrella patens: A Novel Model System for Plant Development and Genomic Studies (Cove et al. 2009a). The growth of protonemal tissue is described in Culturing the Moss Physcomitrella patens (Cove et al. 2009b), and a method for isolation of P. patens protoplasts is found in Isolation and Regeneratio...
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Three cDNA collections covering all important steps of the life cycle of the moss Physcomitrella patens have been used to prepare three normalized and subtracted cDNA libraries for the purpose of mass sequencing. After production of around 110,000 expressed sequence tags a clustered database was built and analysed. The Physcomitrella transcriptome is estimated to contain around 25,000 genes. Wh...
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